2018 Section 6 - Laryngology, Voice Disorders, and Bronchoesophalogy

O. Ortega et al.

Neurogastroenterology and Motility

34 Nadkarni MA, Martin FE, Jacques NA, Hunter N. Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 2002; 148 (Pt 1): 257 – 66. 35 Sanchez M, Darimont C, Drapeau V, Emady-Azar S, Lepage M, Rezzonico E, Ngom-Bru C, Berger B et al. Effect of Lactobacillus rhamnosus CGMCC1. 3724 supplementation on weight loss and maintenance in obese men and women. Br J Nutr 2014; 111 : 1507 – 19. 36 Ortega O, Clave P. Oral hygiene, aspiration, and aspiration pneumonia: from pathophysiology to therapeutic strategies. Curr Phys Med Rehabil Rep 2013; 1 : 292 – 5. 37 Rofes L, Clave P, Ouyang A, Schar- itzer M, Pokieser P, Vilardell N, Ortega O. Neurogenic and oropharyn- geal dysphagia (vol 1300, pg 1, 2013). Ann N Y Acad Sci 2013; 1303 : 105. 38 Connolly MJ. Of proverbs and pre- vention: aspiration and its conse- quences in older patients. Age Ageing 2010; 39 : 2 – 4. 39 CarrionS, CabreM,MonteisR, RocaM, Palomera E, Serra-Prat M, Rofes L, Clave P. Oropharyngeal dysphagia is a prevalent risk factor for malnutrition in a cohort of older patients admitted with an acute disease to a general hospital. Clin Nutr 2015; 34 : 436 – 42. 40 Carrion S, Cabre M, Monteis R, Roca M, Palomera E, Clave P. Association between oropharyngeal dysphagia and malnutrition in elderly patients with acute diseases admitted to a general hospital. Clin Nutr Suppl 2012; 7/S1 : 8. 41 Hiramatsu K, Niederman MS. Health- care-associated pneumonia - A new therapeutic paradigm. Chest 2005; 128 : 3784 – 7. 42 Laforce FM, Hopkins J, Trow R, Wang WLL. Human oral defenses against gram-negative rods. Am Rev Respir Dis 1976; 114 : 929 – 35.

52 Widmer AF, Mertz D, Frei R. Ne- cessity of screening of both the nose and the throat to detect methi- cillin-resistant Staphylococcus aur- eus colonization in patients upon admission to an intensive care unit. J Clin Microbiol 2008; 46 : 835 – 835. 53 Wilson M. Microbial Inhabitants of Humans: Their Ecology and Role in Health and Disease . Cambridge: Cambridge University Press, 2005. 54 Rasmussen TT, Kirkeby LP, Poulsen K, Reinholdt J, Kilian M. Resident aerobic microbiota of the adult human nasal cavity. APMIS 2000; 108 : 663 – 75. 55 Logemann JA, Curro FA, Pauloski B, Gensler G. Aging effects on oropha- ryngeal swallow and the role of dental care in oropharyngeal dysphagia. Oral Dis 2013; 19 : 733 – 7. 56 Scannapieco FA, Bush RB, Paju S. Associations between periodontal disease and risk for nosocomial bac- terial pneumonia and chronic obstructive pulmonary disease. A sys- tematic review. Ann Periodontol 2008; 8 : 54 – 69. 57 Yoneyama T, Yoshida M, Ohrui T, Mukaiyama H, Okamoto H, Hoshiba K, Ihara S, Yanagisawa S et al. Oral care reduces pneumonia in older patients in nursing homes. J Am Geriatr Soc 2002; 50 : 430 – 3. 58 Tada A, Miura H. Prevention of aspi- ration pneumonia (AP) with oral care. Arch Gerontol Getriatr 2012; 55 : 16 – 21. 59 World Health Organization. Denti- tion Status and Criteria for Diag- nosis and Coding (Caries). WHO Oral Health Surveys Basic Meth- ods , 4th edn. Geneva: WHO, 1997: 39 – 44. 60 Coleman P, Watson NM. Oral care provided by certified nursing assis- tants in nursing homes. J Am Geriatr Soc 2006; 54 : 138 – 43.

43 Palmer LB, Albulak K, Fields S, Filkin AM, Simon S, Smaldone GC. Oral clearance and pathogenic oropharyn- geal colonization in the elderly. Am J Respir Crit Care Med 2001; 164 : 464 – 8. 44 Almirall J, Cabre M, Clave P. Com- plications of Oropharyngeal Dyspha- gia: Aspiration Pneumonia. Stepping Stones to Living Well with Dyspha- gia . Basel: Karger, 2012: 67 – 76. 45 Puisieux F, d’Andrea C, Baconnier P, Bui-Dinh D, Castaings-Pelet S, Cres- tani B, Desrues B, Ferron C et al. Swallowing disorders, pneumonia and respiratory tract infectious dis- ease in the elderly. Rev Mal Respir 2009; 26 : 587 – 605. 46 Awano S, Ansai T, Takata Y, Soh I, Akifusa S, Hamasaki T, Yoshida A, Sonoki K et al. Oral health and mor- tality risk from pneumonia in the elderly. J Dent Res 2008; 87 : 334 – 9. 47 Terpenning MS, Taylor GW, Lopatin DE, Kerr CK, Dominguez BL, Loesche WJ. Aspiration pneumonia: dental and oral risk factors in an older veteran population. J Am Geriatr Soc 2001; 49 : 557 – 63. 48 UmedaM, Chen C, Bakker I, Contreras A, Morrison JL, Slots J. Risk indicators for harboring periodontal pathogens. J Periodontol 1998; 69 : 1111 – 8. 49 El-Solh AA, Pietrantoni C, Bhat A, Okada M, Zambon J, Aquilina A, Berbary E. Colonization of dental plaques - A reservoir of respiratory pathogens for hospital acquired pneu- monia in institutionalized elders. Ch- est 2004; 126 : 1575 – 82. 50 Ogawa T, Ikebe K, Enoki K, Murai S, Maeda Y. Investigation of oral oppor- tunistic pathogens in independent living elderly Japanese. Gerodontol- ogy 2012; 29 : E229 – 33. 51 Mertz D, Frei R, Jaussi B, Stebler C, Fluckiger U, Widmer AF. Throat swabs are necessary to reliably detect carriers of Staphylococcus aureus . Swiss Med Wkly 2007; 137 : 56S – 56S.

SUPPORTING INFORMATION

Additional supporting information may be found in the online version of this article at the publisher’s web site: Figure S1 Rarefaction plots. Figure S2 Number of observed species as a function of anatomical niche in all groups of patients. Figure S3 Shannon diversity index for different niches in all groups of patients. Figure S4 Full legend of OTU of Fig. 3 (Microbiota composition of the investigated anatomical locations and groups of patients). Table S1 Microbiota diversity. Table S2 Estimated ratios between all locations for the four groups of patients. Data S1 Supplementary material.

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