Section 4 Plastic and Reconstructive Problems

Rajan, Eubanks, Edwards et al.

Figure 1. Cell-seeding efficiency of b -tricalcium phosphate ( b -TCP). (A): The overall cell-seeding efficiency is shown at different time intervals (15, 30, and 60minutes) following seeding of the scaffoldwith cells. (B): The cell-seeding efficiency at the different time intervals was stratified by the temperature at which the cells were allowed to incubate with the scaffold. (C): SEM images at low and high magnification showing the distribution of cells and cell attachment to one b -TCP particle (individual particle sizes ranged from 500 to 1,000 m m). p , p , .05 relative to the 15-minute condition. Abbreviation: RT, room temperature.

Statistical Analysis Statistical analysis was performed with the use of InStat software (GraphPad Software, Inc., San Diego, CA, http://www.graphpad. com). All data were plotted as mean 6 SEM unless otherwise noted. One-way analysis of variance was performed for the cell-seeding efficiency and cell-viability studies with Bonferroni- corrected, post hoc, two-tailed t tests to determine statistically significant differences between groups. Statistical significance was defined as p , .05.

medications included an antibiotic, a corticosteroid, and an an- algesic for pain management. Four months following placement of the graft, a third CBCT was performed to evaluate the presence of mineralized tissue in the grafted area prior to implant placement. Following the im- aging, the area was surgically re-entered for the placement of oral implants. During re-entry, clinical bone measurements were recorded, and a bone biopsy was performed in an area of the pre- viously grafted region. This tissue biopsywas processed for micro- computed tomography (micro-CT) and histological analysis, as described previously [23]. Oral implants were placed in the grafted sites and allowed to heal underneath the gingival tissue for 6 months. At 6 months, the implants were uncovered, and a dental prosthesis was engineered and installed to connect to the oral implants. This study is registered with ClinicalTrials.gov identifier CT00755911.

R ESULTS Cell-Seeding Efficiency

The time frame needed to achieve the highest cell attachment to b -TCP was determined in our cell-seeding efficiency studies

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