April 2020 HSC Section 4 - Plastic and Reconstructive Problems

Aesth Plast Surg (2015) 39:495–505

by adipose-derived stem cells were significantly increased by the addition of P-PRP [ 11 ]. Atashi et al. showed that culture media supplemented with PRFM promoted greater dose-dependent enhancement of proliferation of adipose- derived mesenchymal stem cells compared to fetal bovine serum; the most effective concentration was found to be 20 % P-PRP [ 12 ]. Amable et al. found that 10 % P-PRP sustained five to tenfold increased proliferation of human mesenchymal cells compared to 10 % fetal bovine serum [ 13 ]. Shin et al. demonstrated that incubating dermal fi- broblasts in 20 % platelet-rich clot (P-PRP) releasate for 48 h markedly up-regulated the expression of both MMP-1 and type I collagen in dermal fibroblasts [ 14 ]. Roubelakis et al. showed that the presence of P-PRP in a fetal mes- enchymal stem cell medium resulted in a significant in- duction of the migration ability and the proliferation rate of mesenchymal stem cells and dermal fibroblasts [ 15 ] (Table 2 ). Twenty-eight animal studies were included in this review. Sclafani et al. showed that wound healing and soft tissue ingrowth into alloplasts could be significantly improved with the use of P-PRP. Subcutaneous PHDPE implants were treated with saline solution, PPP followed immedi- ately with platelet concentrate (PC or P-PRP), or au- tologous blood clot. Compared with controls, statistically significant increases in fibroblast and endothelial cell counts were noted at day 7 in PC-treated implants. They concluded that treatment with PC may be useful in sce- narios in which enhancement and acceleration of early wound healing is desired [ 1 ]. Farrag et al. evaluated the effect of pure-platelet-rich plasma and fibrin sealant on facial nerve regeneration in rats. They compared different methods of facial nerve repair and demonstrated a mea- surable neurotrophic effect when P-PRP was present, with the most favorable results seen with P-PRP added to suture [ 16 ]. Cho et al. showed the effectiveness of P-PRP in re- ducing wrinkles in photoaged in mice. Mice exposed to ultraviolet B irradiation for 8 weeks received saline, P-PRP, or no injection. At 4 weeks, wrinkles in the P-PRP- injected group were significantly reduced compared to the other groups. Biopsy results also indicated that the dermal layer was remarkably thicker and fibroblast proliferation and collagen production were increased in the P-PRP group [ 17 ]. Several animal studies have sought to examine the effect of P-PRP on survival and quality of fat grafts. Out of six animal studies examining the effect of PRP on fat graft survival, five demonstrated a statistically significant im- provement in survival, whereas one did not demonstrate a significant difference. Oh et al. showed improved quality Animal Studies

Table 1 Sixty-one reports reviewed included 13 in vitro studies, 28 animal studies, and 23 case series and clinical trials

In vitro

Animal

Human

Powell et al. [ 41 ]

x

Sclafani et al. [ 1 ]

x

Chandra et al. [ 24 ]

x

Hom et al. [ 42 ]

x

Farrag et al. [ 16 ]

x

Danielsen et al. [ 43 ]

x

Rughetti et al. [ 4 ]

x

Kakudo et al. [ 5 ]

x

Giusti et al. [ 7 ]

x

Por et al. [ 23 ]

x

Mishra et al. [ 8 ]

x

Pires et al. [ 19 ]

x

Nakamura et al. [ 20 ]

x

Sclafani [ 50 ]

x

Danielsen et al. [ 44 ]

x

Redaelli et al. [ 49 ]

x

Giovanni et al. [ 35 ]

x

Kakudo et al. [ 45 ]

x

Cho et al. [ 17 ]

x

Na et al. [ 54 ]

x

Oh et al. [ 18 ]

x

Giovanni et al. [ 36 ]

x

Sclafani [ 3 ]

x

Lee et al. [ 52 ]

x

Rodriguez-Flores et al. [ 21 ]

x

Kim et al. [ 10 ]

x

Kawazoe and Kim [ 33 ]

x

Sclafani [ 2 ]

x

Chignon-Sicard et al. [ 57 ]

x

Shin et al. [ 55 ]

x

Kushida et al. [ 9 ]

x

Miao et al. [ 32 ]

x

Giovanni et al. [ 37 ]

x

Kim et al. [ 30 ]

x

Kakudo et al. [ 6 ]

x

x

Jeon et al. [ 25 ]

x

Yuksel et al. [ 46 ]

x

Mehryan et al. [ 47 ]

x

Willemsen et al. [ 48 ]

x

Gentile et al. [ 51 ]

x

Tajima et al. [ 11 ]

x

x

Li et al. [ 26 ]

x

Atashi et al. [ 12 ]

x

Amable et al. [ 13 ]

x

Shin et al. [ 14 ]

x

Roubelakis et al. [ 15 ]

x

Liu et al. [ 26 ]

x

x

Notodihardjo et al. [ 27 ]

x

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