xRead - Nasal Obstruction (September 2024) Full Articles

20426984, 2021, 3, Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/alr.22741 by Stanford University, Wiley Online Library on [01/07/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

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Orlandi et al.

(Continues)

Decreased structural epithelial barrier function.

Decreased antimicrobial barrier functions.

Decreased structural epithelial barrier function.

antibacterial properties.

Increased MCC and

Effect on epithelial barrier

transepithelial resistance in AFRS.

expression of

Occludin and Junctional

Adhesion

molecule-A. Increased

expression of claudin-2.

SPLUNC1,

LPLUNC2, and

Lactoferrin in CRSwNP.

increase in NP.

OSM Stimulation resulted in

reduced barrier function.

production and mucociliary transport

velocity.

T2Rs Increase NO

OSM OSM expression

Specific gene targets Findings

Decreased

Decreased

Decreased

Adhesion

molecule-A, Claudin-2

LPLUNC2,

Lactoferrin

Junctional

SPLUNC1,

resistance, IHC, Western Blot

Microarray, RT-PCR, ELISA,

Immunoblot, IHC

resistance, RT-PCR

NO production, MCC, bactericidal activity

TABLE IX-16 Evidence for epithelial barrier disturbance as a contributing factor for CRS Study Year LoE Study groups Tissue Techniques Pothoven 848 2015 5 CRSwNP, CRSsNP, Control Mucosa, NP. Epithelial cell culture Transepithelial DenBeste 847 2013 5 AFRSvs Control Epithelial cell cultures Transepithelial

Seshadri 870 2012 5 CRS, Control Mucosaand NP

Epithelial cell culture

Lee 612 2012 5 Primary human nasal cells

genotyped for TAS2R38