xRead - Nasal Obstruction (September 2024) Full Articles

20426984, 2021, 3, Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/alr.22741 by Stanford University, Wiley Online Library on [01/07/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

479

Orlandi et al.

(Continues)

RT-PCR FACS Tissue IL-33 IL-33 was not elevated in NPs. Normal Normal

Ozturan 828 2016 CRSsNP (20) Sinoasal tissue Nasal polyps ELISA Tissue IL-33 IL-33 was not elevated in NPs. Normal Xu 829 2016 CRSsNP (12) CRSwNP (35) Control (12) Sinoasal tissue Nasal polyps RT-PCR IHC Decreased Endo 1504 2015 ECRSwNP Control Increased CRSwNP (20) Control (20)

Increased

Normal

Paris 1507 2014 CRSwNP (8) Sinoasal tissue RT-PCR IHC Tissue IL-33 IL-33 was elevated in CRSwNP. Increased Shaw 1508 2013 CRSsNP (73) CRSwNP (30) Control (8) Sinoasal tissue Nasal polyps RT-PCR ELISA Lam 831 2012 CRSsNP (18) Nasal tissue Increased Control (9)

Normal

Innate Immunity Activity

Sinoasal tissue Nasal polyps RT-PCR Tissue IL-33 The expression level of IL-33 mRNA was not significantly different among the 3 groups. Sinoasal tissue Nasal polyps

RT-PCR Tissue IL-33 The mRNA level of IL-33 were significantly elevated in polyp tissues as compared to the control.

Tissue IL-33 IL-25 mRNA level was significantly lower in the CRSwNP group compared to the control.

RT-PCR IHC Tissue IL-33 The mRNA level of IL-33 were significantly elevated in NP

epithelial cells but not whole tissue as compared to the control. Tissue IL-33 The expression level of IL-33 was not elevated in NPs.

RT-PCR FACS Tissue IL-33 IL-33 was highly expressed in the chronic inflammatory polyps of ECRS patients. Luminex RT-PCR

Tissue IL-33 The expression level of IL-33 mRNA was not significantly different among the 3 groups.

Type of Innate Immunity Findings

Study Groups (size) Tissue Technique

Nasal polyps

Epithelia cells

Nasal polyps

Sinoasal tissue Nasal polyps

Sinoasal tissue Nasal polyps

Non-Eos CRSwNP (33) Control (28)

Miljkovic 1501 2014 CRSsNP (13)

Liao 1500 2015 Eos CRSwNP (28)

Baba 1506 2014 ECRS (10)

NCRS (10)

Control (5)

CRSwNP (12) Control (7)

Steven 1505 2015 CRSsNP (27)

CRSwNP (15)

Control (12)

CRSwNP (7)

Control (32)

TABLE X-13 (Continued) Study Year