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Wise et al.

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Author Manuscript Author Manuscript Author Manuscript Author Manuscript TABLE VIII.F.5. Study

design Study groups Clinical endpoint Conclusion 1 SCIT;

Basophil sensitivity changes correspond to clinical changes in allergy symptoms in patients on SCIT. Basophil reactivity did not change.

BAT using CD203c did not correlate with patient response.

BAT significantly decreased with SCIT; remains decreased 1–2 years after 3–5 years of SCIT treatment. BAT is an objective measure of response to AIT and is a stable marker of allergen response over a long period.

CD-sens 10-fold higher in symptomatic patients. Significant difference between CD63 responsiveness in those with positive NPT vs negative NPT. CD-sens a good predictor of allergic rhinitis symptoms in those sensitized to Timothy grass pollen.

BAT, basophil reactivity. Sensitivity for basophil reactivity 83%–100%; specificity 78%–89%; PPV 75%–87%; and NPV 89%–100%. BAT may replace NPT when NPT is contraindicated. Small numbers of patients used needs to be validated in larger study. CD203c expression on basophils when stimulated with Japanese cedar pollen.

CD203c expression has diurnal variation and should be considered when using CD203c as a marker. This was also shown in basophils derived from marrow of mice-models.

NPT, serum sIgE, BAT. NPT positive in all AR and 10/12 LAR. Serum sIgE positive in AR, negative in LAR. BAT positive in AR and in 8/12 LAR. NPT remains the gold standard, but if unable to be done, BAT should be considered.

Clinical measures of allergy, basophil sensitivity, basophil reactivity.

BAT using CD203c at 2 and 4 months of treatment.

BAT using CD63 as marker for basophil response.

Evaluated after 1st pollen season, after 2nd pollen

season, and 1–2 years after finishing 3–5 years of SCIT. CD-sens, CD63

responsiveness. Tested

before and after pollen season.

2 Open control

Van Overtvelt et al. 978 2011 1b RCT SAR to grass pollen (n = 89); 1 SLIT tablet; 2 Placebo

Zidarn et al. 977 2015 2b Cohort Moderate-severe SAR to grass pollen; 1 SCIT (n = 30); 2 No treatment (n = 20)

Zidarn et al. 976 2012 2b Cohort 1 Positive skin test and sIgE to Timothy grass pollen (n = 26); 2 Positive NPT (n = 13); 3 Negative NPT (n = 13); Lesniak et al. 974 control

4 Nonsensitized healthy controls (n = 10)

Allergy patients (n = 30) diagnosed by clinical symptoms, SPT, or serum IgE. 1 Birch-positive, HDM-negative (n = 15); 1 SAR patients (n = 18); 2 Controls (n = 11)

2 Birch-negative, HDM-positive (n = 15) 1 AR patients (n = 12);

2 LAR patients (n = 12); 3 Controls (n = 12);

SAR to grass pollen (n = 24);

RCT

control

control

Evidence for the use of basophil activation testing in allergic rhinitis Study Year LOE Schmid et al. 971 2014 1b Open

2016 3b Case

Ando et al. 979 2015 3b Case

2015 3b Case

Campo et al. 308

Int Forum Allergy Rhinol . Author manuscript; available in PMC 2020 June 10.