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Wise et al.

Page 347

Skin tests, BAT, histamine release tests, leukotriene production. Significant correlation between skin tests and BAT ( r = 0.72, p < 0.001). Positive and significant correlation between BAT and histamine release tests ( r = 0.80, p < 0.001); allergen-specific LTC4, LTD4, LTE4 production ( r = 0.7, p < 0.001); and the occurrence of serum sIgE ( r = 0.71, p < 0.001). BAT is a

Author Manuscript Author Manuscript Author Manuscript Author Manuscript Study design Study groups Clinical endpoint Conclusion control

BAT, nasal sIgE, NPT. AR: BAT sensitivity 85%, specificity 93%. LAR: BAT sensitivity 50%, specificity 93%. BAT diagnosed at least 50% of cases of LAR to D. pteronyssinus and was more sensitive than detection of nasal sIgE and less time-consuming than NPTs. Discrimination of pollen allergic individuals from controls using CD203c expression as marker of allergy; cutoff values of 14%. Performed during off season.

BAT CD203c can be used to test for grass allergens if conventional measures not available.

CD-sens. CD-sens decreases during early phases of treatment. No change in basophil reactivity. CD-sens good objective measure to use to assess response to AIT. Tested both CD63 and CD203c expression using prescribed protocol.

100% sensitivity for both CD63 and CD203c in cat-allergic patients. CD203 is as reliable as CD63 for diagnosis of patients with IgE-mediated allergy to cat.

No differences in ROC characteristics between tests. BAT can be a useful approach to determine the clinically relevant allergen in sensitized patients.

SAR to grass pollen (n = 26) CD-sens, nPIF. Positive nPIF and positive CD-sens in 92%. Positive nasal symptom scores and positive CD-sens scores in 85%. Subjects tested twice: CD-sens 100% reproducible vs 78% for nasal symptom scores and 94% for nPIF. CD-sens results

highly reliable technique in the diagnosis of allergy to inhalant allergens. BAT sensitivity = 93.3%, specificity = 98.4%, when using a cutoff point of 15% activated basophils as positive result.

reproducible and correlate well with other allergen testing methods. Has potential for diagnosis and follow-up after treatment.

12 patients with AR sensitized to birch or mites Blood sample tested 1, 4, and 24 hours after sampling compared to SPT, sIgE, and NPT.

1 LAR patients (n = 16);

2 AR patients (n = 14);

3 NAR patients (n = 10); 4 Controls (n = 14);

1 SAR to grass pollen (n = 31);

2 Healthy non-atopic controls (n = 9)

1 Patients sensitized to Timothy grass (n = 14);

2 Patients sensitized to birch (n = 19);

1 Cat-allergic patients (n = 20); 2 Controls (n = 19)

1 AR or asthma patients sensitized to HDM (n = 53);

2 AR or asthma patients sensitized to grass (n = 51);

3 Atopic, non-allergic patients (n = 24); 4 Healthy controls (n = 38)

Tested to D. pteronyssinus

Tested to olive tree pollen

Treated with conventional or ultra-rush AIT.

control

control

control

control

series

series

Study Year LOE

2013 3b Case

2011 3b Case

Nopp et al. 969 2009 3b Case

2007 3b Case

Sanz et al. 967 2001 3b Case

2015 4 Case

Nopp et al. 970 2013 4 Case

Ozdemir et al. 972

Ocmant et al. 968

Lesniak et al. 973

Gomez et al. 318

Int Forum Allergy Rhinol . Author manuscript; available in PMC 2020 June 10.